Whole-genome shotgun sequencing of mitochondria from ancient hair shafts
Gilbert, M. Thomas P.; Tomsho, Lynn P.; Rendulic, Snjezana; Packard, Michael; Drautz, Daniela I.; Sher, Andrei; Tikhonov, Alexei; Dalen, Love; Kuznetsova, Tatyana; Kosintsev, Pavel; Campos, Paula F.; Higham, Thomas; Collins, Matthew J.; Wilson, Andrew S.; Shidlovskiy, Fyodor; Buigues, Bernard; Ericson, Per G. P.; Germonpre, Mietje; Goetherstroem, Anders; Iacumin, Paola; Nikolaev, Vladimir; Nowak-Kemp, Malgosia; Willerslev, Eske; Knight, James R.; Irzyk, Gerard P.; Perbost, Clotilde S.; Fredrikson, Karin M.; Harkins, Timothy T.; Sheridan, Sharon; Miller, Webb; Schuster, Stephan C.
Science (Washington, DC, United States) (2007), 317(5846), 1927-1930
CODEN: SCIEAS; ISSN: 0036-8075. English.
Although the application of sequencing-by-synthesis techniques to DNA extracted from bones has revolutionized the study of ancient DNA, it has been plagued by large fractions of contaminating environmental DNA. The genetic analyses of hair shafts could be a solution: The authors present 10 previously unexamd. Siberian mammoth (Mammuthus primigenius) mitochondrial genomes, sequenced with up to 48-fold coverage. The observed levels of damage-derived sequencing errors were lower than those observed in previously published frozen bone samples, even though one of the specimens was >50,000 14C years old and another had been stored for 200 years at room temperature The method therefore sets the stage for mol.-genetic anal. of museum collections.
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