Goldberg, Susanne M. D.; Johnson, Justin; Busam, Dana; Feldblyum, Tamara; Ferriera, Steve; Friedman, Robert; Halpern, Aaron; Khouri, Hoda; Kravitz, Saul A.; Lauro, Federico M.; Li, Kelvin; Rogers, Yu-Hui; Strausberg, Robert; Sutton, Granger; Tallon, Luke; Thomas, Torsten; Venter, Eli; Frazier, Marvin; Venter, J. Craig

Proceedings of the National Academy of Sciences of the United States of America (2006), 103(30), 11240-11245 CODEN: PNASA6; ISSN: 0027-8424. English.

Since its introduction a decade ago, whole-genome shotgun sequencing (WGS) has been the main approach for producing cost-effective and high-quality genome sequence data. Until now, the Sanger sequencing technol. that has served as a platform for WGS has not been truly challenged by emerging technologies. The recent introduction of the pyrosequencing-based 454 sequencing platform offers a very promising sequencing technol. alternative for incorporation in WGS. In this study, we evaluated the utility and cost-effectiveness of a hybrid sequencing approach using 3730xl Sanger data and 454 data to generate higher-quality lower-cost assemblies of microbial genomes compared to current Sanger sequencing strategies alone.